Background: Transition is the period of learning, adjustment and socialization, when the nurse applies, consolidates and increases their existing knowledge, gaining competence (knowledge, skill, and attitude) that is applicable to the nursing practice of the clinical setting or patient population in which they are expected to perform. Aim: investigating effect of effect of transition training program on novice bachlors' degree nurses' working at Minia University Hospitals. Research design: Quasi-experimental research design was utilized in this study. Setting: The study was carried out at Minia University Hospitals. Subject: A convenient sample the study subjects were include all novice nurses (bachelor's degree) in University Hospitals. They are working in the field of nursing practice less than one year (no=110). Tools: Two tools were used in this study; nurse's transition training program knowledge questionnaire, and The Casey-Fink Graduate Nurse Experience Survey. Results: There were statistical significance differences in knowledge test scores as well as mean scores of transition role among new graduate nurses. Conclusions:  There was a positive effect of transition training program on Novice Nurses. Recommendations: Further attention to academic preparation of nursing new graduate nurses to promote their perceptions about role transition.