Background: Intravenous therapy is an important part of the treatment of many hospitalized patients. Fluid and electrolyte therapy is an essential component of the care of hospitalized children. The aim of this study is to assess pediatric nurses' performance regarding intravenous therapy in Minia University hospital and Minia General Hospital.  Design: Descriptive research design was used for this study. Setting: This study was conducted at Minia University for Pediatrics and Obstetrics Hospital and Minia General Hospital affiliated to the Ministry of health and population. Subjects: A convenient sample consisting of 58 nurses who were divided into 29 nurses from the pediatric and emergency department at Minia University Pediatrics and Obstetrics Hospital as well as 29 nurses from the pediatric and emergency department at Minia General Hospital over six months were included. Tools of data collection: Three tools were included for data collection; Tool І: A structured interview questionnaire sheet in an Arabic language. It consisted of two parts: Part 1: personal data of nurses as age, qualifications, years of experience, and previous training courses. Part 2: Knowledge assessment sheet: it contained 30 close-ended questions to assess nurse's knowledge about nursing care regarding insertion, maintenance, and removal of the intravenous line. Tool II: Observational checklist sheet was developed by the researcher to assess the nurses' practices for intravenous therapy it contained 12 procedures (149 steps) Tool III: Nurses' attitude regarding intravenous therapy. More than half of nurses in Minia University Hospital (MUH) and 44.8% of them in Minia General Hospital (MGH) had incomplete knowledge regarding intravenous therapy with high statistical significance differences P-value .006.  Also, more than two-thirds of nurses in MUH and 51.7% of them in MGH had incomplete practice regarding intravenous therapy with no statistical significance differences P-value .117. Conclusion: These results concluded that one third of nurses worked in Minia University Hospital had very good knowledge and 10.3% of them had complete practice than a few of  nurses worked in Minia General hospital but more than three quarters of nurses in Minia general hospital had positive attitude regarding intravenous therapy with statistically significant difference which P-value 0.04. Recommendations The education program should be applied and repeated every 3 months in the same study setting and adopted in other similar settings with necessary modifications, the provision of continuing education programs is suggested regularly to refresh and update the knowledge of nurses, as well as to reinforce appropriate practices in pediatric units related to intravenous therapy with continuous supervision.