Background: Cardiopulmonary resuscitation (CPR) is an emergency procedure for a person whose heart has stopped or no longer can breathing, CPR save live. Nurses' role is vital in resuscitation process. Aim of the study: to Evaluate effect of educational protocol about resuscitation process on nurses' performance. Research design: Quasi - experimental research design utilized to fulfill the purpose of this study. Subjects: A Purposive sample included sixty nurses from critical care units. Setting: This study was carried out at five critical care units affiliated to Minia University Hospitals- Eygpt. Tools of data collection: Two tools designed and used for collecting data for this study, First: structured questionnaire sheet to assess nurses' knowledge, second: Observational checklist for nurses′ practice sheet regard resuscitation process. Results: There was a highly statistically significant difference in nurses' knowledge and practice pre and post educational protocol regarding resuscitation process. Also, there was no statistically significance between personal data and their knowledge level or practice score.  Conclusion: Based on the results of this study educational protocol can increase nurses′ performance about resuscitation process. Recommendations: Greater attention and closely observation from hospital on nurse's performance during resuscitation process. Replication of the study should be done on a large sample.