Background: Health care organizations cannot succeed without productive nursing staff. In order to enhance efficiency in health interventions, improving the productivity and performance of health care workers is a major challenge. Performance of health care organizations depends on the knowledge, skills and motivation of individual employees. Aim of the study: To identify nursing staff perception regarding factors influencing their performance at Minia General Hospital. Design: Descriptive research design was utilized. Setting: The study was conducted at all departments of Minia General Hospital in Minia city. Sample: All nursing staffs who working at Minia General Hospital was included. Tools: Data was collected using one tool which was modified by researcher. Results: The nursing staffs of Minia General Hospital perceived the most factors influencing on their performance were; organization policies, work environment and supplies, reward effect, management style, work relation, job design influence and the last one was training and development opportunities. Also, there were highly positive statistically significant correlations between personal data regarding age and experience with training and development; in addition the reward effect had a highly statistically significant correlation with most performance factors and its dimensions except work relation factor and work environment and supplies. Conclusion: The majority of nursing staffs at Minia General Hospital had “moderate level" of perception about the whole factors influence on their performance. Recommendation: Developing and implementing training and development programs are necessary to acquire skills and knowledge about their competence which has a positive effect on staff performance.