Background: the core of nursing education program is the clinical education in which training of nursing student occur to have competent nurse student. Also, clinical instructors play a significant role in creating positive learning experiences in the clinical setting that shape student behaviors at the work environment. Therefore, clinical instructors should have the effective clinical teaching behaviors to create a positive learning environment for nursing students. Aim: to assess the clinical instructor's behaviors as perceived by themselves, students and Nursing Faculty Staff. Research Design: The study was conducted using descriptive research design. Setting: The study was carried out at faculty of Nursing, Minia University. Subject: The study subjects consisted of all clinical instructors, staff members and about 80% of students at (2nd, 3rd, 4th year) at the Faculty of Nursing, Minia University. Tool: The Nursing Clinical Teacher Effective Inventory (NCTEI) was used for data collection. Results: revealed that, the high level of clinical instructors behaviors were from clinical instructors themselves view with (89.2%), followed by students view (68.2%) and then view of staff members (59.2%) with statistically significant difference between clinical instructors, students and staff members view (P=0.001). Conclusions: Clinical instructors had biased self-assessment regarding perception of their clinical teaching behaviors. Recommendation: Workshops/seminars should be organized and also orientation program for all newly clinical instructors on their roles in clinical teaching.