ManageChatDiscover
202208

Emergence of resistance to last-resort antibiotics in clinical isolates of staphylococci with special reference to daptomycin and linezolid

Article

Last updated: 05 Jan 2025

OverviewSimilar Items

Subjects

-

Tags

chemotherapy

Abstract

Background: Treatment of staphylococcal infections has been complicated by the continuous emergence of antibiotic-resistant strains. Objective: In this study, we investigated the resistance pattern of clinical isolates of both coagulase-positive and coagulase-negative staphylococci to antibiotics recently introduced to treat staphylococcal infections. Methodology: Minimum inhibitory concentrations of antibiotics were determined by agar dilution or broth microdilution method. Identification of daptomycin- and linezolid-resistant isolates was performed by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Moreover, the mechanism of resistance to daptomycin and linezolid was investigated on the molecular basis using PCR and amplicon sequencing. Results: Out of 104 staphylococci, 9 were resistant to ciprofloxacin (8.7%), 68 to clindamycin (65.4%), 2 to daptomycin (1.9%), 54 to erythromycin (51.9%), 6 to linezolid (5.8%), 3 to nitrofurantoin (2.9%), 75 to oxacillin (72.1%), 37 to teichoplanin (35.6%), 69 to tetracycline (66.3%), 51 to tigecycline (49%), and 41 to vancomycin (39.4%). Identification of daptomycin- and linezolid-resistant isolates revealed that they belong to Staphylococcus aureus, S. hominis, S. capitis, and S. epidermidis. In addition, sequencing of the mprF gene conferring daptomycin resistance revealed L431F and S829L point mutations in the two resistant isolates identified as S. aureus and S. hominis, respectively. Furthermore, linezolid resistance was due to optrA gene in two, and cfr in three of the resistant isolates. Conclusion: Resistance to the last-resort antibiotics used to treat staphylococcal infection has emerged. Therefore, the use of daptomycin and linezolid should be restricted to critical cases not susceptible to other available agents.

Keywords

Antibiotic-resistance, Daptomycin, Linezolid, Nitrofurantoin, Staphylococci, Tigecycline

Authors

First Name

Nouran

Last Name

Assar

MiddleName

Hamed

Affiliation

Department of Microbiology, General Division of Basic Medical Sciences, Egyptian Drug Authority (EDA), Formerly National Organization for Drug Control and Research (NODCAR), Giza, 12611, Egypt

Email

drnouranhamed@hotmail.com

City

Giza

Orcid

-

Volume

30

Article Issue

1

Related Issue

28170

Issue Date

2021-01-01

Receive Date

2020-12-16

Publish Date

2021-01-01

Page Start

191

Page End

199

Print ISSN

1110-2179

Online ISSN

2537-0979

Article File

EJMM_Volume 30_Issue 1_Pages 191-199.pdf

PDF . 486.6kB

Link

https://ejmm.journals.ekb.eg/article_202208.html

Detail API

https://ejmm.journals.ekb.eg/service?article_code=202208

Order

24

Type

New and original researches in the field of Microbiology.

Type Code

2,038

Publication Type

Journal

Publication Title

Egyptian Journal of Medical Microbiology

Publication Link

https://ejmm.journals.ekb.eg/

MainTitle

Emergence of resistance to last-resort antibiotics in clinical isolates of staphylococci with special reference to daptomycin and linezolid

Details

Type

Article

Created At

23 Jan 2023