Amarek's disease virus (MDV) strain isolated locally from an apparently healthy bird was attenuated in chicken embryo fibroblast cell culture. The virus at its 20th passage level was used as a vaccine and the protect ion offered was compared with MD attenuated vaccine (Poultry Biologic als) and Herpes virus of turkey (HVT) F or strain. All the 3 vaccines showed significant protection against challenge with the classical strain of MDV. The degree of protection was highest in MD attenuated (Poultry Biologicals) strain i.e. 95% followed by HVT vaccine (92%) and local isolate of MD attenuated vaccine (87.5%). The vaccinated as well as vaccinated challenged birds showed gain in body weight comparable to controls. The virus reisolation was less in the vaccinated challenged birds.