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185555

COMPARISON OF CONVENTIONAL STAINING TECHNIQUES AND FITC-LABELED MONOCLONAL ANTIBODY-BASED METHOD FOR DETECTION OF CRYPTOSPORIDIUM OOCYSTS IN NEONATAL CALVES

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Last updated: 05 Jan 2025

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Abstract

This study was assessed to discover the ability of four staining techniques, initially, developed for the rapid identification of Cryptosp < /em>oridium sp < /strong>ecies oocysts in faecal samples of 55 neonatal calves less than 2 weeks old. All the stains employed in this study (modified Ziehl-Neelsen, Safranin-methylene blue, Giemsa and FITC-labeled monoclonal antibody as a commercial kit) detected the oocysts with incidence discrepancy. Concentration flotation of the samples raised diagnostic value of all techniques used. FITC labeled monoclonal antibody was the best in identifing the oocysts from concentrated faecal samples (72.7%) as well as from the unconcentrated one (63.6%). The recovery of oocysts by other methods was variable includes modified Zeihl-Neelsen (45.5%), safranin-methylene blue (36.4%) and Giemsa stain (18.2%). Our findings suggested that FITC-labeled monoclonal antibody as a probex to detect the oocyst specific epitope even without concentration flotation was highly sensitive and specific than the other traditional techniques. Combination between fluorescent antibody and modified Ziehl-Neelsen stains, where possible, are the currently recommended procedures for the detection of oocysts.

DOI

10.21608/avmj.1994.185555

Keywords

monoclonal Comparison, conventional staining FITC antibody based method, cryptosporidium oocyst, calves

Authors

First Name

G.H.,

Last Name

SALEM

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Orcid

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First Name

M.G.,

Last Name

NESSIEM

MiddleName

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Affiliation

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Orcid

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First Name

R.S.,

Last Name

YACOUP

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-

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Volume

32.1

Article Issue

63

Related Issue

26616

Issue Date

1994-10-01

Receive Date

1994-08-22

Publish Date

1994-10-01

Page Start

45

Page End

53

Print ISSN

1012-5973

Online ISSN

2314-5226

Article File

AVMJ_Volume 32.1_Issue 63_Pages 45-53.pdf

PDF . 2.4MB

Link

https://avmj.journals.ekb.eg/article_185555.html

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https://avmj.journals.ekb.eg/service?article_code=185555

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Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

COMPARISON OF CONVENTIONAL STAINING TECHNIQUES AND FITC-LABELED MONOCLONAL ANTIBODY-BASED METHOD FOR DETECTION OF CRYPTOSPORIDIUM OOCYSTS IN NEONATAL CALVES

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Article

Created At

23 Jan 2023