The prevalence of Campylobacter jejuni and Campylobacter coli harboring in the intestine of calves and lambs with or without diarrhea was studied. A total of 309 rectal swab samples were collected from calves (167) and lambs (142) with and without diarrhea, the samples were examined bacteriologically. The bacteriological examination revealed the isolation of C. jejuni from 24 (14.37% of 167 examined calves. The prevalence was significantly higher in the diarrheic calves (23.4%) than from the apparently healthy calves (10.83%). In addition, C. jejuni was isolated from 13 (9.15%) of 142 sheep examined, where higher (but non significant) percentage (12.24%) was reported in diarrheic lambs than (7.52%) reported in apparently healthy lambs. Also, lower prevalence of C. coli was observed in examined samples of calves (8.98%) and lambs (7.04%). The prevalence of C. coli was significantly higher in diarrheic calves (17.82%) than apparently healthy calves (6.67%), and higher in diarrheic lambs (8.16%) than apparently healthy lambs (6.45%) (Non significant). Moreover, Campylobacter isolated in this study were tested for susceptibility to 9 antibiotics. None of the isolates were resistant to Chloramphenicol and high percentages of isolates were resistant to Tetracycline, Erythromycin, Ampicillin, Penicillin, Naldixic acid and Sulfamethoxazole, while drug resistance was more frequent in C. coli than C. jejuni. These results indicate that Campylobacter colonization in the intestine is very common in young calves and lambs and the bacteria play a role, at least in the aggravation of the diarrhea process. Additional studies are needed to assess the role of campylobacter in spontaneous enteric disease of calves and lambs. The results of this study demonstrate that C. jejuni is widely distributed, while C. coli is more narrowly distributed but significantly more resistant to antimicrobial. The public health importance of C. jejuni and C. coli were discussed.