A total of (120) samples of aborted foeti, vaginal discharage and Placenta were collected from (70) aborted ewes and (50) aborted she goats from Menoufiea Governorate for bacteriological and mycological examination. Swabs from stomach and intestinal contents of aborted foeti as well as liver , spleen, and lungs were collected. The bacteriological examination of aborted foeti, vaginal discharage and Placenta of aborted sheep revealed the isolation of Brucella melitensis, Campylobacter fetus subsp.fetus, Campylobacter fetus subsp.venerealis, Listeria monocytogens,  Salmonella typhimurium, Salmonella dublin, Escherichia coli and Staph. aureus with the incidences of 21.4%, 11.4%, 7.1%, 8.6%, 5.7%, 2.9%, 1.4% and 1.4%, respectively. While the bacteria isolated from aborted she goats were Brucella melitensis, Campylobacter fetus subsp. fetus, Campylobacter fetus subsp.venerealis, Listeria monocytogens,   Salmonella typhimurium, Salmonella dublin, Staph. aureus and Escherichia coli with the incidences of 20%, 10%, 6%, 10%, 8%, 4%, 4% and 2%, respectively.                   Mycological examination of aborted foeti, vaginal discharage and Placenta of aborted sheep revealed the isolation of Aspergillus fumigatus, Aspergillus niger, Aspergillus flavus, Candida albicans , Candida krusei, Mucor spp. Abisidia spp. and Rhodotrula spp. with incidence of (12.9%,5.7%, 2.9%, 8.6%, 2.9%, 4.3%, 2.9% and 1.4%, respectively). While the fungi isolated from aborted she goats were Aspergillus fumigatus, Aspergillus niger, Aspergillus flavus, Candida tropicalis, Candida albicans, Mucor spp., Rhizopus spp., Penicillium pp. and Fusarium spp. with incidence of (14%, 6%, 2%, 10%, 8%, 6%, 4%, 2% and 2%, respectively).          The stomach contents of the aborted foeti of ewes and she goats were the most common seat for the isolation of bacteria and fungi which cause abortion             In vitro the antibiogram test indicated that the different bacterial species were more sensitive to danofloxacin, gentamicin, erythromycin and amoxicllin & clavulanic acid. While the most fungal isolates were sensitive to clotrimazole and  miconazole.       PCR (Polymerase chain reaction assay) was a valuable tool for direct and rapid diagnosis of Brucella melitiensis andAspergillus fumigatus from aborted foeti specimens. The amplification of 169 and 792 bp fragments from the extracted DNA of Brucella melitensis, while 383 bp fragments from the extracted DNA of Aspergillus fumigatus were done.