In the present study two groups of stray adults and puppies dogs were captive in small boxes for special drug trial. After 3 months the dog attendants complained with gastrointestinal disturbances, diarrhea, weakness, headache and fever. In the same time, some dogs are suffering from diarrhea also. Investigation of these cases revealed infection by several pathogens isolated from both attendants and dogs. Entamoeba histolytica, Giardia (trophozoites & cysts) and Isospora species oocysts were diagnosed in feces of 20%, 20% and 26.6% respectively in attendants closely contact to dogs. Anti-Toxocara canis, anti-Sarcocystis and anti-hydatid cysts antibodies were diagnosed in 20%, 33.3% and 46.66% respectively in sera of attendants using EL1SA. Puppies and dogs were infected by Toxascaris leonina (20% & 16.66 %), Dipylidium caninum (53.33% & 66.66%) and Taenia spp. eggs (26.6% & 50%). Giardia (53.33%), E. histotytica (13.33%), Toxocara canis (33.3%), Isospora (53.3%) and Cryptosporidium oocysts (20%) were diagnosed in puppies only. Two adult dogs (16.66 %) are shed Sarcocystis oocyst and Ancylostoma caninum eggs in their feces. Bacteriological examination of fecal swabs evidenced infection by 3 enteric bacteria in attendants, puppies and dogs. Salmonella (20%, 33.3% & 41.66%), Campylobacter (13.3%, 33.3% & 33.3%) and enterotoxigenic E.coli (46.66%, 46.66% & 58.33%). Serotyping of these bacteria revealed presence of S. typhimurium in dogs (60%) and attendants (66.6%), S.enteritidis in one of the worker as well as 8 untyped strains. Two serotypes of C. jejuni in 2 workers and 4 dogs, E. coli in 3 dogs, while 2 untyped isolates were recorded in dogs. Three serotypes of E. coli (O26, O 76, O 55) and two untyped strains were isolated from workers and dogs. Moreover two isolates (O 5 & O 111) were isolated from dogs only. The isolates showed high sensitivity for Gentamycin(l0 µg). The study recommended some precautions to minimize the role of captive dogs as a potential source of zoonotic pathogens