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ISOLATION OF S. ENTERITIDIS AND OTHER SALMONELLAE FROM CREAM AND IT'S STABILITY AGAINST SORBATES AND HONEY

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Last updated: 05 Jan 2025

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Abstract

100 random samples of raw and whipped cream (50 samples each) were tested for the presence of S. enteritidis and other Salmonella spp. From raw cream 15 and 14 initial Salmonella isolates were recovered using S.S. and Bismuth sulphite agars, respectively. Only 2 isolates were identified as Salmonella spp. on both media on the basis of modefication of FDA. Regarding whipped cream, 13 and 4 presumptive Salmonella colonies were isolated on the same media, of these, 3 and 2 isolates were identified as Salmonella spp on both media respectively. The stability of S. enteritidis against potassium sorbate or honey in cream stored at refrigerator temperature was studied. Cream inoculated with 1x107 S. enteritidis, devided into 10 parts to which potassium sorbate was added, in concentrations of 0.2, 0.4 and 0.6%. Fennel honey was added in concentrations of 0.2, 0.4, 0.6, 1, 5 and 10%. One part was kept as control. The samples were examined for S. enteritidis count and pH in the 1st and 2nd day then, every 2 days of storage. Lower decrease in count of S. enteritidis was noticed in cream containing 0.2, 0.4 and 0.6% pot .sorbate, stored at refrigerator temperature. Undetectable numbers of     S. enteritidis were observed at 10th day in concentration of 0.6%. While, in control samples the count reached 8x107 in the 1st day then, decraesed to be 2x105 at the end of the storage time. In contrast, addition of honey at conc. of 10% inhibits the growth of S. enteritidis within 24 hours of storage at refrigerator temperature. Lower concentration of honey (1 and 5 %) led to appearance of injured colonies in the 1st and 2nd day, the colonies begin to recover at the 4th day, and no viable cells were noticed after the 10th day. Gradual reduction in the count of S. enteritidis using 0.2, 0.4 and 0.6% honey was observed till the 10th day. Our results showed which preservative is most active against S. enteritidis, thus, the safety of cream could be improved by addition of fennel honey in a concentration of 10%.   key wo

DOI

10.21608/avmj.2009.169787

Keywords

cream, Salmonella, sorbates, Honey

Volume

55

Article Issue

120

Related Issue

24384

Issue Date

2009-01-01

Receive Date

2008-11-18

Publish Date

2009-01-01

Page Start

7

Page End

22

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_169787.html

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https://avmj.journals.ekb.eg/service?article_code=169787

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2

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

ISOLATION OF S. ENTERITIDIS AND OTHER SALMONELLAE FROM CREAM AND IT'S STABILITY AGAINST SORBATES AND HONEY

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Article

Created At

23 Jan 2023