In this study, the effect of black leg disease was detected on cattle's meat in middle region's slaughterhouses in Syria at which meat's goodness and its suitability for human's use and its inspection decision were evaluated. This research was applied on samples from cattle carcasses (75) suspected to be infected by black leg disease in Hama's slaughterhouse (35) samples, Homs's slaughterhouse (25) samples, and Rastan's slaughterhouse which appendant to Homs (15) samples. The pathogenic agent (Clostridium chauvoei) was isolated from Hama's slaughterhouse only in one sample while in Homs and Rastan slaughterhouses we haven't any positive sample. The sensitivity test for eight antibiotics on positive sample to delimit the suitable antibiotic to treat black leg disease was carried out.