Beta
168755

AMELIORATIVE EFFECT OF SPIRULINA PLATENSIS AGAINST DICLOFENAC SODIUM-INDUCED HEPATOTOXICITY IN BROILERS: HEMATOLOGICAL, BIOCHEMICAL, ANTIOXIDANT, IMMUNOLOGICAL AND HISTOPATHOLOGICAL STUDY

Article

Last updated: 23 Jan 2023

Subjects

-

Tags

-

Abstract

Spirulina is one of the most commonly used dietary supplements in humans, aquaculture, aquarium, and recently in poultry industries and gained more attention to be used as a nutritional and therapeutic strategy. The present study has been carried out to investigate the effect of oral supplementation of Spirulina platensis in alleviating the hepatotoxic effect of diclofenac sodium through evaluating the hematological, biochemical, antioxidant parameters as well as immunological and histopathological studies. One hundred broiler chicks were divided into four groups (25 per each). The first group was fed on basal diet and treated with propylene glycol (2.5 mg/kg, i.m) (Control negative). The secondgroup was fed on basal diet and injected with diclofenac sodium at dose (2.5 mg/kg.b.wt., i.m) (Control positive). The third group was injected with diclofenac sodium and then concurrently supplemented with Spirulina at dose (10 gm/kg in their diet). The fourth group was fed on Spirulina two weeks before injection of diclofenac sodium at dose (2.5 mg/kg, i.m). Haematological data revealed that diclofenac-treated chicks showed normocytic normochromic anemia, significant leucopenia and decrease the absolute number of heterophils and lymphocytes. Significant increase in serum biochemical parameters related to liver injury such as AST and ALP, total cholesterol and significant decrease in the levels of serum total proteins and albumin. Significant increase in uric acid level was also noticed. Furthermore, significant increase of oxidative damage biomarkers such as MDA and NO and significant decrease of the antioxidant enzymes as CAT, SOD and TAC. Moreover, diclofenac sodium has immunosuppressive effect through depressed phagocytic activity, phagocytic index and marked decrease in the titer of antibodies formed in chicks against Newcastle disease. Histopathological findings marked hepatic tissue damage due to gouty tophi lesions accompanied with presence of multifocal necrotic areas. Oral supplementation of Spirulina markedly decrease the histopathological alteration on preventive basis. Therefore, Spirulina supplementation markedly alleviated the deteriorating effects of diclofenac sodium-induced hepatotoxicity which mimics the gouty lesions in birds either on therapeutic or preventive regimes.

DOI

10.21608/avmj.2018.168755

Keywords

Key words: Spirulina, antioxidant, diclofenac sodium, Hepatoprotective

Authors

First Name

ABD-ALLAH

Last Name

MOKHBATLY

MiddleName

-

Affiliation

Clinical Pathology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt

Email

-

City

-

Orcid

-

First Name

EMAD

Last Name

GHAZY

MiddleName

-

Affiliation

Clinical Pathology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt

Email

-

City

-

Orcid

-

First Name

DOAA

Last Name

ABDELHADY

MiddleName

-

Affiliation

Department of Clinical Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt.

Email

doaa_assar2000@yahoo.com

City

-

Orcid

-

First Name

ABDO

Last Name

WALIED

MiddleName

-

Affiliation

Pathology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt.

Email

-

City

-

Orcid

-

First Name

MARWA

Last Name

ABDELWAHAB

MiddleName

-

Affiliation

Clinical Pathology Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Egypt

Email

-

City

-

Orcid

-

Volume

64

Article Issue

156

Related Issue

24312

Issue Date

2018-01-01

Receive Date

2017-11-07

Publish Date

2018-01-01

Page Start

164

Page End

177

Print ISSN

1012-5973

Online ISSN

2314-5226

Link

https://avmj.journals.ekb.eg/article_168755.html

Detail API

https://avmj.journals.ekb.eg/service?article_code=168755

Order

22

Type

Research article

Type Code

1,840

Publication Type

Journal

Publication Title

Assiut Veterinary Medical Journal

Publication Link

https://avmj.journals.ekb.eg/

MainTitle

-

Details

Type

Article

Created At

23 Jan 2023