Background: Direct pulp therapy is a technique used for the treatment of the traumatic or mechanical pulp exposures. Removal of irritation, control of infection and biocompatibility of capping material are important factors in treatment. Aim: This study aimed to evaluate the histopathological effects of different antiseptics materials: normal saline, sodium hypochlorite, 3 mix antibiotics on healing processes of direct pulp capping. Materials and Methods: The study carried on 54spraug dwelly rats that were divided into three groups, 18 rats in each group that placed in three cages. The study were conducted on upper (right or left) first molar teeth of each rat. Every 6 rats within each group were treated with 0.9% sterile saline, 0.5% sodium hypochloride (NaOCL), and 3MIX-MP, respectively. In each group, rats were serially sacrificed at 7 days, 30 days and 60 days. Specimens from tooth and periapical tissue were fixed in buffered formalin and processed for histopathological evaluation. Results: The results revealed that groups treated for 3 mix were significantly improved inflammatory response and proper tissue healing of teeth pulp and periapical tissue on short and long time, in the contrary normal saline and sodium hypochlorite. Conclusions:This study confirmed the effectiveness of 3 mix as a strong antibacterial, on short and long duration, and haven't toxic effect on pulpal tissue or periapical tissue, this allow the success of direct pulp capping.   Keywords: Overdentures, copy post with different length, retention test, different type cement.