The present study aimed to monitor the antioxidant and protective effects of Spirulina platensis (SP) against Nano- copper particles (NCPS) which induced liver injuries in rabbits. The experimental animals were randomly divided into three groups, the first group, (control), the second group, (received NCPS, 37.5 mg/kg), and the third group, received NCPS, 37.5 mg/kg bw plus SP, 1.5 g/kg diet for 2 months. After ending the period of the experiment, rabbits were decapitated and, blood, liver, as well as muscle tissue samples were collected for examination of liver function, antioxidant activity, lipid peroxidation, and copper levels. Exposure of rabbits to these Nanoparticles S\C for 8 weeks resulted in significant increase levels of glutathione (GSH) in the third group. While hepatic superoxide dismutase (SOD) and malondialdehyde (MAD) activity showed a significant decrease in their levels of the same group. In addition, alanine aminotransferase (ALT) in serum showed a significant decrease. These data revealed that administration of SP protects against Nano copper-induced hepatotoxicity through amelioration of hepatic damage and oxidative stress in rabbits.