Background: Biogas is produced by methanogen bacteria during acting upon the degradable materials in an anaerobic condition. Aim of study: Was to evaluate effect of Health education program of the household owners of biogas units and the employees at the biogas station, for knowledge and practices regarding Negative effect of Biogas Units. Research Design: A quasi -experimental design was utilized in this study. Setting: The study was conducted at biogas units and special units of society situated in recycling center at Moshtohor village, Kualubiya Governorate. Sample: A convenient sample, it consisted of 118 workers (number of owner (23) and number of employees (95)). Tools of data collection: Two tools were used in this study. I:Interviewing sheet included two parts: A); Demographic characteristic of the workers, owner biogas unites, B); Current history of occupational injury or problem and workers' knowledge owners of biogas units about biogas health hazards. II: Observational checklist: Was consisted of two parts: A); Observing and assessing of working place or condition. B); Observing and assessing household owners of biogas units and working employees in the biogas station practices during preparing biogas production. Results: Less than two thirds (60.9 %) of the studied owner their age ranged from 40 to less than 50 years old. More than half (54.7 %) of the studied workers their age ranged from 40 to less than 50 years old. revealed that there was improvement in workers, owner biogas unites total knowledge about score and total practice. Conclusion: Studied biogas owner and workers' knowledge and practice were improved significantly post program. Recommendation: Educational intervention and awareness programs for workers and biogas owner   regarding   safety measure and negative effect of biogas. Should be provided in wide score.