Collaboration is an important concept in healthcare and particularly in nursing as effective nursing collaboration and teamwork contributes to improved nursing performance The purpose of the study was to assess the effect of implementing workshops for nurses to improve their knowledge and skills about collaboration and their effect on their performance at Menoufia University Hospital and National Liver Institute. Research Design: A quasi-experimental design was utilized to achieve the purpose of the current study. Sampling: All available head nurses (17) and simple random sample of staff nurses (154) working in selected units. Setting: Medical and surgical intensive care units at Shebin El-Kom Menoufia University Hospitals and National Liver Institute in Menoufia Governorate, Egypt. Instruments:  Five instruments were utilized; namely; collaboration knowledge questionnaire, head nurse / nurse collaboration scale,nurse/nurse collaboration scale,observational checklist for performance of staff nurse and observational checklist for performance of head nurse. The results: There was a positive improvement in studied head and staff nurses' knowledge, skills regarding collaboration and nursing performance after implementation of workshops. Conclusion: The study concluded that there was a positive effect of the workshops for head and staff nurses on their knowledge, skills regarding collaboration and performance. Recommendations: Future studies recommended to investigate the effect of nursing collaboration on other variables such as nurses' turnover, absenteeism, and productivity.