Evidence-based practice in the health care setting has become a gold standard and a core competency for all health care professionals. The purpose of the study was to investigate the nurse managers' knowledge and skills of evidence based nursing practice. Methods: Descriptive design was used. The study was conducted at Menoufia University hospital and Shebin El-Kom Teaching hospital. A convenient sample was used to select 90 nurse managers. Two instruments were used for data collection. Knowledge' and skills assessment questionnaires were used for data collection. Results revealed that mean score of nurses according to their perceived knowledge of evidence-based nursing practice was 44.80% in Shebin El Kom Teaching hospital vs. 48.49% in Menoufia University hospital. Mean score of their related perceived skills was 46.21% vs. 53.22% in Shebin El Kom Teaching hospital and Menoufia University hospital. Conclusion: Approximately half of the nurses did not perceive that they have enough knowledge and skills about evidence based practice. Recommendation: The study results should be disseminated to the important key persons (nurse managers) to be considered.