Background: Blood sampling withdrawal is the most frequently procedure performed to neonates at neonatal
intensive care unit (NICU). In order to overcome the mortality and morbidity caused by nurses' malpractices that
happen during blood sampling withdrawal we need to look at this research point. Aim: this study aimed to assess
nurses' malpractices during blood samples withdrawal at NICU. Method: A descriptive study was conducted in NICU
at Mansoura University Children Hospital on (80) nurses. Data were collected using structured interview
questionnaire and observational checklist. Results: revealed that, over half of the studied nurses' total practical
knowledge regarding blood samples withdrawal was unsatisfactory and more than one quarter of the studied nurses'
total practices regarding blood samples withdrawal were incompetent. Conclusion: the majority of the studied nurses
were lacking knowledge and the minority of them had incompetent practice toward blood samples withdrawal.
Recommendation: the study emphasizes on the necessity to train and educate the neonatal nurses in NICU on best
practices about blood sampling procedure to get the best in health care of neonates.