Pelvic organ prolapse is considered as one of the most common causes of reproductive morbidity which influences the women's quality of life. Aim: The present study aimed to assess women's knowledge regarding pelvic organ prolapse. Subjects and Method: The study followed a descriptive design on 220 women with pelvic organ prolapse who were chosen by convenient sample technique. Setting: The study was conducted at Outpatient Gynecological Clinics in the Obstetrics and Gynecology Center at Mansoura City, Egypt over a period of six months from July 2017 to the end of December 2017. Data collection tools: Two tools were used for data collection including; A structured interviewing questionnaire and pelvic organ prolapse knowledge questionnaire. Results: The study revealed that majority of women had lack of knowledge regarding pelvic organ prolapse. Recommendations: The study recommended the importance of developing pelvic organ prolapse guidelines aiming for improving women's knowledge and awareness regarding symptoms and proper management.