Stigma is a key problem for individuals with mental illness, which is a negative label through which the community reacts adversely towards the mentally ill. Persons with mental illness may internalize mental illness stigma and experience diminished self-esteem and self-efficacy so this study aims to assess the impact of psycho-educational Program on mentally ill patient's stigma and self-esteem. It has been carried out using a quasi- experimental research design. The subjects were constituted of 50 psychotic patients divided into 25 study group and 25 control groups from in-patient Psychiatric Department of Mansoura University Hospital. In order to collect the necessary information for the study structure interview sheet was used to collect data. Data collection was conducted during the period from February 2017 to July 2017. There was a significant positive correlation between internalized stigma and externalized stigma also there was a significant negative correlation between self-esteem, internalized stigma and externalized stigma so it is important to continue stigma psycho-educational program to overcome its negative consequences.