Background: Central venous catheter (CVC) represents one of the intravascular access devices that are necessary for critically ill patients. However, it is associated with several risks, of these are central line associated bloodstream infections (CLABSIs) which are serious but often preventable when evidence-based guidelines such as central line bundle are followed. Aim of the study: To assess nurses' knowledge, and practices about central line care bundle at an Emergency Hospital, Mansoura University. Research design: A design was descriptive exploratory. Sample: A convenience sample consisting of 64 nurses was included. Tools of data collection: Data were collected through two tools: critical care nurses' knowledge assessment questionnaire and nurses' practice observational checklist. Results: The great majority of nurses had unsatisfactory knowledge and practices regarding central line care bundle in percentages of 85% & 89.7% respectively. Unsatisfactory knowledge was found in relation to central line associated blood stream infection and central line bundle in percentages of 87.9% & 74.1% respectively. Unsatisfactory practice was found regarding utilization of insertion and maintenance bundle in percentages of 91.4% & 89.7% respectively. Conclusion: nurses had unsatisfactory knowledge and practice. Recommendations: Enhancement of nurses' knowledge and practices regarding central line care bundle through carrying out training programs and ntitepeper of this study on a larger sample.