Background: Nursing is a stressful profession that deals with human aspects of illness and health. It requires the provision of human, empathetic, skilled and ethical care, in work environment with increasing responsibilities and restricted resources. The inequity between providing high-quality care and managing the stressful work environment can lead to burnout. Aim: The aim of this study was to study the relation between work environment and nurses' burnout at Mansoura Health Insurance Hospital. Research design: A descriptive correlation design was used. The study was conducted at Mansoura Health Insurance Hospital. Subjects of the study: Subjects were included (196) nurses. Two tools were used for data collection, work environment scale, and burnout inventory. Results and conclusion: Findings revealed that 49.5 % of nurses had negative perception regarding their work environment and 34.7% had a moderate level of burnout. The correlation between work environment and burnout was negative. Recommendations: Improving nursing work environment and management of burnout through providing a supportive environment, clarifying roles and responsibilities, and providing regular training courses for the nurses on coping strategies.