Background: Hepatitis C virus (HCV) is one of the major causes of liver disease. It is one of the leading causes of cirrhosis and hepatocellular carcinoma, and is a common indication for liver transplantation. The aim of study: To assess health care workers' knowledge, attitude and practice toward caring of patients with hepatitis c. Materials and methods: The study design used was a descriptive cross sectional design. A convenient sample of 227 health care workers was selected. Tool used divided to four parts including demographic data, and questions related to study subjects' knowledge, attitude and practice toward hepatitis C. The results of this study: Revealed that there was a positive statistical significant correlation between attitude and knowledge of the studied health care workers. Conclusion: Physicians were the most knowledgeable and less than half of nurses had satisfied knowledge. Recommendation: Providing Continuous educational programs for health care workers about hepatitis C to keep them up to date with HCV.