Background: The most common bacterial infection during pregnancy is urinary tract infection, and it is the primary cause of many complications for both mother and fetus. As a result, effective management based on correct knowledge and more satisfying self-care practices are crucial to prevent these issues. The study aimed to examine the effect of applying self-efficacy nursing guidelines on pregnant women's performance regarding urinary tract infections. Method: The researcher used a quasi-experimental research design. A purposive sampling of eighty pregnant women with urinary tract infections was enrolled to study and control groups, each with forty women. The researcher conducted the research at Shebin El-Kom, Menoufia Governorate's Maternal and Child Health Centers (Quibli and Bahari). The researchers used a structured interview questionnaire, a self–care evaluation interviewing schedule, and an assessment of urinary tract infections' symptoms recovery. Results: Before applying the guidelines, more than forty percent of the pregnant women had poor knowledge and unsatisfactory performance related to self-care of urinary tract infections. In addition to two weeks and three months after the intervention, the study group had a statistically significant increase in total knowledge scores (seventy-five and sixty-five percent, respectively had good knowledge scores) and perceived self-efficacy in performing self-care practices (seventy-five and seventy-nine percent, respectively had satisfactory scores) to relieve urinary tract infection symptoms in contrast to before. However, the difference was not noticeable in the control group. Also, there was a highly statistically significant difference in the recovery of urinary tract infection symptoms as reported by pregnant women after applying the nursing guidelines. Conclusion: The self-efficacy nursing guidelines have been a successful intervention in increasing the pregnant women's knowledge and performance related to self-care of urinary tract infections to relieve symptoms of urinary tract infections. Recommendations: During antenatal follow-up visits, all pregnant women should follow the self-efficacy nursing guidelines concerning healthy practices to reduce urinary tract infections and avoid related maternal and fetal complications.