COVID-19 pandemic remains a worldwide challenge. Healthcare providers including maternity nurses fighting on the frontlines. Issues as knowledge, awareness of mode of disease transmission, basic hygiene principles and measures will help support them and safeguard lives of everyone. Aim of this study: to evaluate the effect of educational program on nurse's knowledge, practice and attitude regarding Covid 19 at maternity care units Subjects and Methods: Quasi- experimental study design was used; study was conducted at Minia university hospital for maternity and child. A convenient sample of seventy nurses was included. Data were collected using a structured interviewing questionnaire sheet contained a series of questions regarding nurses knowledge, attitude, and practice (KAP) about COVID-19. In addition to, a supportive material (guidelines) was distributed to the studied sample. Results: This study revealed that nurses had lower knowledge scores (32.9%) in pre-program which increased to (90.0%) in post-program after the program, (58.6%) of them had negative attitude pre-program while post-program decreased to (18.6%) and (84.3%) had incompetent practice pre-program while post-program decreased to (18.6%) with highly statistically significance differences P – value .0001. Conclusion: applying educational program about COVID-19 for maternity nurses was effective in increasing their knowledge, improves infection control measures, change negative attitudes, and improve practices. Recommendations: Apply further wide range educational programs for whole nursing staff, nursing supervisors, and workers.