The present study was carried out in the farm of the Department of plant Production, Faculty of Environmental Agricultural Sciences, Arish University. This study was carried out to investigate the effect of feeding Carniolan honey bee on different supplementation on venom amounts collected and some biological activities under North Sinai conditions. Results indicated that pollen grains diet significantly (P≤0.05) recorded the highest bee venom weight (0.1631 and 0.2106 mg/col.) during the spring season of 2015 and 2016 years, respectively. However, the lowest bee venom weight (0.0811 and 0.0692 mg/col.) was observed in the control diets during the spring season of 2015 and 2016 years, respectively. Results indicated that pollen grains diet significantly (P≤0.05) recorded the highest bee venom weight (0.1724 and 0.1567 col.) during the summer season of 2015 and 2016 years, respectively. Pollen grains diet significantly (P≤0.05) recorded the highest bee venom weight (0.0924 and 0.0653 col.) during the autumn season of 2015 and 2016 years, respectively. Results revealed pollen grains diet significantly (P≤0.05) recorded the highest bee venom weight (0.1515 and 0.1544 mg/col.) during 2015 and 2016 years, respectively compared with other treatments.  Results  of the interaction between protein nutrition and seasons on bee venom weight  indicated that honeybees fed pollen grains diet significantly (P≤0.05) recorded the highest bee venom weight (0.1515 and 0.1544 mg/col.) during 2015 and 2016 years, respectively compared with other treatments. Pollen grains diet significantly  recorded the highest honey stored area, sealed brood area and pollen stored area (746, 777.5 and 106.3 inch²/colony) during spring season of 2015 year, respectively and (987.8, 593.6 and 140.8 inch²/colony) in the 2016, respectively. Results revealed that the bee colonies fed pollen grains diet significantly (P≤0.05) recorded the highest honey stored area, sealed brood area and pollen stored area (878.6, 390.4and 123.4 inch²/colony) during summer season of 2015 years, respectively and (1233.73, 525.3 and 118.6 inch²/colony) in the 2016, respectively. Results indicated that the bee colonies fed pollen grains diet significantly (P≤0.05) recorded the highest honey stored area, sealed brood area and pollen stored area (504.3, 145.8 and 67 inch²/colony) during  autumn season of 2015 years, respectively and (825..8, 363.8 and 363.8 inch²/colony) in the 2016, respectively. Results revealed that the bee colonies fed pollen grains diet significantly (P≤0.05) recorded the highest honey stored area, sealed brood area and pollen stored area (731.4, 427.7 and 102.5 inch²/colony) in the 2015 years, respectively and (1024.2, 595.2 and 111 inch²/colony) in the 2016, respectively. The data for the interaction between protein nutrition and seasons biological activities indicated that, the bee colonies fed pollen grains diet in spring, seasons significantly recorded the highest honey stored area, sealed brood area and pollen stored area (746.8, 593.6 and 106.3 inch²/colony) in the 2015 years, respectively and (987.8, 777.5 and 140.8 inch²/colony) in the 2016, respectively.