the purpose of this study was identifying the biomechanical and biochemical variables related to muscle fatigue for athletics of different running. Twelve athlete participate in this study (age=19.2 ± 0.8 y; height=179.4 ± 2.9 cm; mass=68.9.2 ± 10.9 kg). All subjects signed an informed consent document before the experiment. Each player performed two trials of a continuous vertical jump for 60s (VJ) with a day of rest between each trial. Bertec force plate (500Hz) was used to collect vertical ground reaction force and (Megawin ME 6000 wireless system, Mega Electronics Ltd., Finland) was used for electromyography analysis. Quadriceps Rectusfemoris (RF), vastuslateralis (VL), vastus medialis (VM) biceps femoris (BF) Gastrocnemius muscle - lateral part (GL) Gastrocnemius muscle - medial part (GM) Tibialis anterior muscle (TA) and Soleus muscle (S) were selected for EMG analysis. Root mean square (RMS) used to rectify the signal and the Area under signal was calculated for each muscle to determine the percent of contribution to the performance (work/loading). Jumping height increases due to the increase of strength in short running runners with decreased rate noted for other running. Although the mechanical performance is very similar and muscle contribution percentages are very close, there are significant differences between the three groups of running on physiological fatigue enzymes.