The problem of gender discrimination remains a major barrier to human development, and specifically the discrimination occurring in the workplace. Discrimination can be defined as those negative and inequitable actions intended to limit or gainsay the equal treatment of a person and group (Allport, 1954). This research paper investigates the effect of employee's gender in being vulnerable to receive discrimination at workplace, particularly at KAAUH.  We hypothesized that there is a gender discrimination occurs at the workplace and happening for the women employees more than men. Data for this research were collected using a cross-sectional questionnaire. The study was conducted on employees from KAAUH, Saudi Arabia. Sociodemographic characteristics which include gender, and age, and general information, which is position, and department were collected. The used scale was Workplace Gender Discrimination scale and it's consisted of six-items. The research findings suggest that there is a significant effect of employees' gender on being vulnerable to discrimination at workplace and women were attaining higher scores of gender discrimination compared with men. In contrast, position and age groups were having no significant influence on the gender discrimination level. It's concluded that, there is a gender discrimination occurs in workplace. Particularly, the female employee is more likely to be discriminated due to her gender than men do at workplace.