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69793

BINDING OF PIPRINHYDRINATE TO PLASMA PROTEINS AND THEIR SUBSTITUTES

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Last updated: 04 Jan 2025

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Abstract

The binding of piprinhydrinate to bovine serum albumin (BSA) was studied in isotonic Sorensen's phosphate buffer pH 7.4 at 25±1°C, using equilibrium dialysis technique. Piprinhydrinate was bound to BSA and the plot was curved indicating the presence of morethan one binding site on the albumin molecule. At fixed protein concentration the percentage of bound drug to bovine serum albumin was inversely proportional to the concentration of the drug. But the binding parameters (binding constants as well as the number of binding sites) were found to be unaffected as the albumin concentration increased. However, binding parameters showed no significant difference due to pH change. Phosphate buffer, Gomori's tris-HCl buffer and Walpole's acetate buffer have different effect on the binding of piprinhydrinate to BSA; the values of the binding parameters can be arranged as follows: Sorensen's phosphate buffer > Gomori's tris-HCl  > Walpole's acetate buffer. Also, it was found that chloride ions decrease the amount of the drug bound to BSA. The interaction of the drug with dextrans (40000, 266000 and 500000) in isotonic Sorensen's buffer pH 7.4 was investigated. No interaction was observed between the drug and the tested dextrans under the condition of the experiment. Also laevosan and hetastarch showed no significant interaction.

DOI

10.21608/bfsa.1994.69793

Authors

First Name

M.

Last Name

Mohamed

MiddleName

S.

Affiliation

Department of Pharmaceutics, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt

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Orcid

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First Name

F.

Last Name

Gazy

MiddleName

S.

Affiliation

Department of Pharmaceutics, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt

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City

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Orcid

-

First Name

S.

Last Name

Abu-Zaid

MiddleName

S.

Affiliation

Department of Pharmaceutics, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt

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City

-

Orcid

-

First Name

B.

Last Name

Müller

MiddleName

W.

Affiliation

Department of Pharmaceutics, Institute of Pharmacy, Kiel, Germany

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City

-

Orcid

-

First Name

M.

Last Name

Abd ElRahman

MiddleName

M.

Affiliation

Department of Pharmaceutics, Faculty of Pharmacy, Zagazig University, Zagazig, Egypt

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Volume

17

Article Issue

2

Related Issue

10609

Issue Date

1994-12-01

Receive Date

1994-05-26

Publish Date

1994-12-31

Page Start

95

Page End

103

Print ISSN

1110-0052

Online ISSN

3009-7703

Article File

BFSA_Volume 17_Issue 2_Pages 95-103.pdf

PDF . 524.5kB

Link

https://bpsa.journals.ekb.eg/article_69793.html

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https://bpsa.journals.ekb.eg/service?article_code=69793

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Original Article

Type Code

1,096

Publication Type

Journal

Publication Title

Bulletin of Pharmaceutical Sciences Assiut University

Publication Link

https://bpsa.journals.ekb.eg/

MainTitle

BINDING OF PIPRINHYDRINATE TO PLASMA PROTEINS AND THEIR SUBSTITUTES

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Article

Created At

22 Jan 2023