To ascertain the frequency of pathogenic bacteria in Yemeni poultry meat Maeen Area-Sana'a. This study aimed to determine the general microbiological quality characteristics and presence of Citrobacter spp. in chicken meat samples obtained from butcher shops. One hundred Fifty (150) chicken meat samples were collected randomly from local markets in Sana'a city from January to April. 2022. Based on using differential and selective media, bacteria were isolated from the samples and characterized by using biochemical tests. Vitek 2 compact was used to confirm the diagnosis. The results of this study showed that, out of 150 poultry meat samples, 140 samples (93% ) were positive and 10 samples (7 %) were negatively isolated. Among 302  isolates: Citrobacter spp 106 (35.1 %), Klebsilla spp 55 (18.2 %), Proteus vulgaris 42 (13.9 %), Sigella spp 36 (11.9 %), E. coli 27 (8.9 %), Proteus mirabilis 24 (8%) and Salmonella spp 12 (4 %). The study showed that the raw chicken meat samples of Sana'a city, Yemen, were contaminated with Gram-negative potential pathogenic bacteria. We must use new methods of testing and characterization for bacteria. PCR-based methods for identifying pathogens provide more advantageous options for this purpose than conventional testing.