Background: Prevention of infection is a challenge for health-care systems, especially at labor and delivery care units. The aim of this study was to evaluate the effect of educational program on nurses' performance regarding infection control at labor and delivery care units. Subjects and method: Design: A quasi-experimental research design. Setting: Labor and delivery care units of Tanta University, as well as El-Menshawy and Zefta General Hospitals, and Al-Santa Central Hospital. Subjects: All available nurses (170) working at the previously mentioned settings were included. Tools for data collection: Tool (I): Structured Interview Schedule for Nurses that included socio-demographic characteristics, and nurses' knowledge regarding Infections Control. Tool (II): Infection Control Observation Checklist that included items related to nurses' practice, and to labor and delivery care units. Results: There is a statistically significant effect of the educational program on nurses' performance regarding infection control at labor and delivery care units. Conclusion and recommendations: Nurses' performance regarding infection control at labor and delivery care units significantly improved due to the educational program. Thus, conduction of in-service training programs at labor and delivery care units is important to enhance nurses' performance.