Background: Quality care is the right of all patients and the responsibility of all nurses to gain desired outcome by using innovative behavior for generating new ideas directed to apply implement new knowledge or improved processes to reach best result. Aim: To determine the effect of quality care program on nurses' innovative behavior at Tanta Ophthalmic Hospital. Subjects and Method: Setting: the study was conducted at Tanta Ophthalmic Hospital affiliated to Ministry of Health and Population. Subjects: Consisted of all available (N=80) ophthalmic nurses who provide direct care to patients at Tanta Ophthalmic Hospital. Tools: Data were collected using four tools. Structured Knowledge Questionnaire about Quality of Care and Innovative Behavior, Ophthalmic Nurses' Quality Care Observational Checklist, Ophthalmic Nurses' Commitment and Improvement Self-Report and Innovative Behavior Assessment Scale. Results: 78.8% of ophthalmic nurses had poor total knowledge level of quality care that improved to be majority (90.3%) of them had good total level of knowledge at immediately post program. Also, there was statistically significant correlation between ophthalmic nurses' innovative behavior and all quality care dimensions except for involvement of family and continuity of care dimensions. Conclusion: Implementation of a designed program about quality care dimensions and innovative behavior lead to significant improvement in ophthalmic nurses' knowledge about quality care and innovative behavior. Recommendations: Integrate quality care dimensions in ophthalmic hospital policy, encourage nurses to generate innovative ideas, support application of these ideas, and follow barriers hinder its applicability.