{Background: Eclampsia proceeds to be a major health problem, particularly in developing countries that, adding significantly to high maternal mortality and morbidity rate. Maternity Clinical Pathways (MCP) is one of the main tools used to manage the quality of health care concerning the standardization of care processes. The implementation of these tools lead to reduce the variability in clinical practice and improves outcomes. Aim: The study aimed to evaluate the effectiveness of clinical pathway application on improving nurses' knowledge and practice in managing eclamptic women. Subjects and Method: Design: A quasiexperimental design was utilized (one group pretest-posttest and follow up).Settings: This study was conducted at gynecology and obstetrics departments affiliated to University Hospital and Shebin El-Kom teaching hospital, Menoufia Governorate, Egypt. Subjects: A purposive sample of 60 maternity nurses. Tools: Three instruments were used for data collection: A structured interviewing questionnaire, Assessment tool of nurses' knowledge, and an observational checklist to assess maternity nurses' practice regarding care of eclamptic women before and after clinical pathway application. Results: The study revealed that there were statistically significant differences before and after implementation of the clinical pathway manifested by higher knowledge and practice score of the maternity nurses. Recommendations: Application of the clinical pathway for maternity nurses is very important to improve their knowledge and practice. In addition, facilitations and barriers of the application of clinical pathway should be analyzed to ensure an effective patient outcome