Background: Patients' rights have become the center of national attention in the practice of nursing which are integral components of human rights. These rights are derived from medical professional values and code of nursing ethics, to defend patients' rights and ensure nondiscrimination while providing care. Objective: The aim was to determine effect of training program on technical nursing interns' performance regarding application of patients' rights. Design: Quasi experimental research design was used. Setting: Study was conducted at medical department at Kafr ELShiekh general hospital affiliated to Ministry of Health. Subjects: All technical nursing interns (n=50) and all patients (n=133) admitted within six months were participated in the study Tools: Three tools were used; (1) Technical nursing interns' knowledge questionnaire (2) Technical nursing interns' practice observation checklist (3) Patients' satisfaction interview. Results: Technical nursing interns range (60% - 54%) showed either poor or fair knowledge level about all domains of patients' rights which improved post program to be range (86% - 76%) have good level of total knowledge. Technical nursing interns range (66% - 56%) had unsatisfactory level of patients' rights practice preprogram, changed post program to be range (88% - 78%) had satisfactory practice level. Post program 81.2%, 74.4% and 63.9% of patients had high satisfaction level on access to information and emergency services, respectful and nondiscriminatory services and keep privacy, and confidentiality domains. Conclusion: The designed and implemented training program significantly improved technical nursing interns total knowledge and maintain their practices about patients' rights. Recommendation: Technical nursing interns required to attend training program about patients' rights prior to internship training to update their knowledge.