Background: Promoting the level of knowledge of patients through education is one of the effective factors in increasing the patient responsibility in eliminating or changing unhealthy behaviors. Also, improving self-management of hemodialysis patients needs empowering the patients through educational program. Aim: to assess the effect of structured teaching program on knowledge and self-management behaviors for hemodialysis patients. Subjects and method: Design: A quasi-experimental (pre- post-test) design was used. Setting: The study was conducted in hemodialysis unit at Qena General Hospital. Subjects: A convenient sample consisted of 100 adult patients were selected based on the following inclusion criteria; their age between 18-65 years, and able to provide oral consent they were followed up for three months. Tools: Three tools were used for data collection; (I) patient's assessment sheet, (II) the hemodialysis knowledge questionnaire and (III) the hemodialysis self-management instrument. Results: Main results for this study showed that only 17% of studied patients had satisfactory level of knowledge in pretest, which increased to 93% in post-test. There was a statistically significant difference as regard hemodialysis self-management behaviors post implementing the structured teaching program P- value <0.001*. Recommendations: Continuous educational programs should be planned and offered to patients on regular basis in hemodialysis units. Also, further studies about home self- management of hemodialysis patients should be conducted.