Breast cancer is the most frequently occurring cancer in women. One out of every 10 women have breast cancer in their lifetime and 1 out 3 have a risk of dying from breast cancer. The early diagnosis of breast cancer increases the rate of survival. Many tumors can be detected by the women herself by breast self-examination. Aim of the study: this study was carried out to evaluate the effect of Breast Self Examination educational program on knowledge, attitude and practice of female employees. Setting: This study was conducted in faculty of nursing Menoufia University and the old secondary school for girls in Menoufia governorate. Sample: A Convenient sample, consisted of (60) female employee and agree to participate in this study. Tools: two tools were utilized for data collection. 1. Interviewing Questionnaire. 2. Champion's Health Belief Model Scale (1997). The results revealed that there was a significant difference between before and after implementation of the educational program of breast self examination in relation to nurses' knowledge, attitude and practice of breast self examination. Conclusion: In the current study the researcher concluded that the implementation of the educational program of breast self examination was effective in improving females' knowledge, attitude and practice of breast self examination. Recommendation: An educational program about breast self examination should be designed for all women in any age groups and presented in mass media.