A field experiment was conducted in South Sinai Government at wadi Sudr Experimental Station, Desert Research Center, in a calcareous sod during the winter growing season of 2001 - 2002. The aim of this experiment was to evaluate the effect of gypsum as a soil amendment and bio-ferblization on the production of sugar beet grown under calcareous soil and saline irrigation water conditions. The experiment was carried out in a split plot design, where the main plots received gypsum (in two rates; 0 and 100 % of gypsum requirements of the sod). Meanwhile four treatments of bio-fertilizers, i.e. control, Elio-N (N-fbdng dazatrophs), Bio- P (phospho-bacterein "PDB") and mixed treatment (Bio-fl + Bio-P) were the sub plots. Results showed that, the yields of roots and leaves increased significantly due to the adation of gypsum or bio-fertilizers, while the percent of total soluble solids (TSS) significantly decreased. The concentrations and uptake of N, P, K, Mn, Zn and Fe of roots and leaves were increased with the addition of gypsum or No-fertilizers. The highest rate of increment in the uptake of these nutrients was associated with the combined treatment of (Gyp. (+) + (810-N + Bio-P)). On the other hand, soil pH values as well as concentrations of soluble Na and CI were decreased in the gypsum or No-fertilizers treated plots. Opposite trend was obtained for EC ESP values as well as concentration of soluble Ca^**, Mg'', e, FICO3 and SO4. The interaction between gypsum and biufertifeers indicated that gypsum amendment in presence of bio-fertilizers Is more effective in improving soil chemical properties as shown in reducing soil pH, EC and ESP values. In addition, the addition of gypsum and No-fertilizers led to increase the values of total N, available P, Mn, Zn and Fe. The combined treatments (Gyp. (+) + (Bio-N + Bio-P)] had the highest effect on increasing Azotobader and Nos*, Hum counts among all other treatments.In conclusion, the application of gypsum with inoculating the Sugar beet seeds with biNfertilizers (Blip-N + Bio-P) c be recommended to increase the calcareous soil productivity of sugar beet when using saline water in Irrigation.