A new Egyptian cotton variety Giza 97(Gossypium barbadense L.) accompanied by superior yield, fiber quality and resistance to Fusarium wilt (Fusarium oxysporum f. sp. vasinfectum) is developed by cotton breeding Section, Cotton Research Institute, Agricultural Research Center, Giza, Egypt. The new variety achieves targets for farmers (high yield, early maturity, resistance to pests and diseases), trader (high lint percentage), and spinner (good fiber quality). Also, had higher water use efficiency by saving 20% of irrigated water. Giza 97 originated from a cross between germplasm line [(Giza 89 x R101) x Giza 86] and Giza 94. Starting from F2 generation, the selection of a single plant was done using pedigree selection method. The superior combinations were finally selected at F5 generation based on yield potential, fiber quality over the commercial varieties. Multilocations yield trials showed that these advanced lines had higher yield productivity plus maximum stability over five locations during two growing seasons and widely adapted in Delta region. During eight growing seasons Giza 97 was increasing significantly over the two commercial varieties (Giza 94 and Giza 86) over all locations The new variety characterized by 12.35 K/F, 13.93 K/F and 39.82% for seed cotton yield, lint yield and lint % traits. While, the fiber quality traits were characterized as a long staple cotton variety such as fiber length (33.8 mm), uniformity index (85.6 %), micronaire value (4.4), fiber strength was (44.6 g/tex), yellowness or +b (8.9) and yarn strength (2400) as the average mean during three growing seasons.