Background: The maternity nurse's role is to promote and preserve the health of the mother and fetus during childbirth. Oxytocin is a dangerous drug that must be closely monitored to avoid negative effects on the mother and fetus and to assess labor progress. Aim: identify the effect of educational program on maternity nurses' knowledge and practices regarding oxytocin drug administration during labor. Methods: Quasi experimental research design was used in this study. It was conducted at Women Health Hospital, Assiut University in places that deal with oxytocin drugs. Sample included 144 maternity nurses. Two tools was used a structured interview questionnaire that involved two parts (personal data and knowledge assessment sheet) and observational checklist for practices sheet. An educational program was implemented to maternity nurses on oxytocin drugs. Results: there is highly statistical significance difference between pre &posttest regarding total knowledge and practices of studied maternity nurses regarding oxytocin drugs p-value 0.001 for both. Conclusion: The knowledge of maternity nurses about oxytocin drug improved between the pretest and posttest as evidenced by the pretest and posttest knowledge scores. Recommendations: provide a continuous education and training program to maternity nurses' on oxytocin drugs.