Abstract: Oocyte cryopreservation is an essential means for assisted reproductive technology; it can markedly contribute to advances in infertility treatment and reproductive biology. Aim: To evaluate the impact of educational guideline on nursing students' knowledge, beliefs and attitudes toward oocyte cryopreservation. Research design: quasiexperimental pretest/posttest research design was utilized. Sample: a cluster randomized sample include 125 female students at Faculty of Nursing - Minia University, from the 1st, 2nd, 3rd and 4th academic level that include 47, 33, 22, 23 respectively. Tools: data were gathered via three tools: the first include socio-demographic data and knowledge assessment tool, the second include beliefs assessment tool, and the third attitudes assessment tool regarding oocyte cryopreservation. Results: 55.2 % of studied students had poor knowledge scores in pretest reduced to 17.6% of them in posttest with highly statistically significant difference. Furthermore, 60.0% of them had negative beliefs about egg freezing in pretest vs. 33.6% in posttest with statistically significant difference. Additionally, only 25.6% of them had positive attitude about egg freezing in pretest increased to 56.8% in posttest with statistically significant difference. Conclusion: There were highly statistically significant improvements in students' knowledge, beliefs and attitudes about oocyte cryopreservation after implementation of educational guideline; Recommendation: Conducting health education programs about oocyte cryopreservation on a large sample in non-medical faculties to increase their awareness about it.