Abstract   Leader should direct subordinate creativity all of the time, to help them to become more creative and accept organizational change. This study was aimed to assess the effect of Nurse Leaders' behaviors on nurses' attitude toward change at Main Assiut University Hospital. The study subjects consists of (48) nurse leaders and (328) staff nurses who worked in medical, surgical, and ICUs units. Data collected through personal interview by using the demographic data sheet, leader's behaviors questionnaire, and organizational change questionnaire, the data collection took about four months.   Reliability was high more than 0.9 for all tools. Results of the study showed that; Nurse leaders in high level, about near half of them have positive attitudes toward organizational change and about third of them have negative and neutral attitudes toward organizational change in low level of leaders behaviors. And staff nurses abouthalf of them have positive attitudes toward organizational change in high leader behaviors and about third of them have neutral attitude toward organizational change in low leader behavior. There were a highly statistically significant difference between predominant organizational change with leader behavior among staff nurses. In the light of the study results, the following conclusions can be drawn; Nurses' leaders and staff nurses had positive attitude toward change and there are a negative correlation between leaders behaviors and attitude toward organizational change. In the light of the finding, the researcher recomended that: provide psychological support to motivate nurses to use their opinions and suggestion without fear, provide training programs to develop leaders and nurses' knowledge which helps in accepting the change.