Aim: this study aimed to explore the knowledge levels, practice and attitudes of nurses toward patients HBV /HCV. Methods: A quantitative descriptive correlation study design was utilized to identify the nurses' performance and attitude, the study was carried out in tropical medicine and gastroenterology departments at Assiut University Hospital. Sample: A convenience sample including nurses as (10) males and (55) females. Tools utilized for data collection were structured interview questionnaire sheet to assess their knowledge and attitudes, observation checklist sheet to evaluate nurses' practice. Results: showed that, about half of the nurses had age > 30 years, while the majority of them were females. As regarding level of education, two third of the study group had diploma degree and about half of the study group their experiences were > 3 years. In addition, 46.16% of nurses their knowledge poor, 30.77% passed, 15.38% good and 7.69% very good. Regarding nurse's practices the results show that 76.9 % of nurses were unsatisfactory .Overall, nurses expressed negative attitudes. Conclusion: Study findings concluded that, discriminatory attitudes are common among nurses, half of nurses had poor comprehensive knowledge and the majority of study nurses their practices were unsatisfactory. Recommendations: further studies are needed to focus on service educational program for nurses, there must be standardized written nursing care and develop strategies for improving public health knowledge