Abstract Aims: To assess nurses knowledge and practice regarding cystectomy patients and develop postoperative nursing care standards for patients undergoing cystectomy at South Egypt Cancer Institute. Subject & Methods Descriptive research design was utilized in this study. Sample: A convenient sample of (60) nurses male and female working in surgical oncology department. The tools were utilized for data collection includes the following: Nurse's knowledge questionnaire, Nurses practice observation checklist, and Construction of standard of nursing care: Post-operative nursing care standards. Results: The majority of nurses are female (86.7%), more than half of nurses their age ranged from 20 >30 years (71.7%), and had diploma of nursing (70.0%). The majority of nurses are married (80.0%) and no had attended training programs (80.0%, 95.0 % respectively). More than two third of nurses (55.0%) years of experience from 5-10 years. Conclusions: Nurses` knowledge and practice regarding postoperative nursing care standards for patients undergoing cystectomy in surgical oncology department at South Egypt Cancer Institute are inadequate that necessitate developing nursing care standards for such group of patients. Recommendation: Nurses are needs for in-service training programs and refreshing courses to improve their knowledge which will reflect into their practice regarding postoperative nursing care standards while working with patients.