Abstract viral hepatitis remains a serious problem and a public health priority in Egypt and major cause of morbidity and mortality. Aims of the study: To determine the seroprevalence of both HCV and HBV infection among infertile couples and assesses knowledge regarding HCV and HBV infection. Methods: A cross-sectional research design carried out in Women's Health Center at Assiut University hospital from client in Gynecology department. Purposive sample included a total of 200 infertile couples according the budget from Assiut University. Data collected by using; tool (1): structured interviewing questionnaire for assessment knowledge of couples and included demographic data and infertility, medical and surgical history, Tool (2): serological blood tests to detect infection with HCV and HBV. Results: The mean maternal age of females and males was (29.2±9.8 & 34.5±11.3 respectively), the prevalence of HCV was 8% (4.5% females and 3.5% males) and prevalence of HBV was 4% (1% females and 3% males) and Majority of couples had poor knowledge regarding HCV and HBV. Conclusion: There is a significant poor knowledge of infertile couples toward Hepatitis B and C. Recommendation: need more researches for a larger number of people from different geographical areas to increase awareness about HBV and HCV.