Abstract Patients with systemic lupus erythematosus are suffering from a lot of problems that can be overcomed by nursing education that focused on the improvement of patients' knowledge level, self-care. This study aimed to evaluate the effect of nursing education on knowledge level and self-care for patient's with systemic lupus erythematosus. Research design: This study was pre-post test research design was be utilized in this study. Sample: This study carried out on (200) patients with SLE, adult patients from both sex, their ages are ranged from (20-65) years old and are willing to participate in the study. Setting: The study was conducted in internal medical department (Rheumatology unit) at Assiut University Hospital. Tools: Two tools were used: patient assessment sheet, pre-post knowledge level and Self-Care questionnaire. Results: There were a statistical significant difference between total scores of knowledge level, self-care pre and post-test (after one month, after three month) after implementing nursing education (P=0.001). Conclusion: designed nursing education protocol were effective in improving knowledge level and self-care for patient with systemic lupus erythmatosus. Recommendation: Conducting additional studies on larger samples from a wider range of participants.