Background: Ante partum hemorrhage (APH) is a grave obstetrical emergency and is a leading cause of maternal and perinatal mortality and morbidity, and nurses play a major role in the care of women with APH. Aim: assess maternity nurses' knowledge about APH, and evaluate effect of educational program on improving maternity nurses' knowledge regarding APH. Methods: Quasi experimental research design was used in this study. It was conducted at Women Health Hospital, Assiut University in places that deal with APH patients. Sample included 86 maternity nurses. Structured interview questionnaire was used and included two parts. An educational program was implemented to maternity nurses on APH. Results: there is highly statistical significance difference between pre &posttest regarding total knowledge of studied maternity nurses regarding APH p-value 0.001. Conclusion: there was an improvement in the knowledge of maternity nurses regarding APH as evidenced by the pretest and posttest knowledge scores. Recommendations: provide a continuous education and training program to maternity nursing staff on APH.