Background: Handover facilitate the safe transfer of patient information in organization, the transfer of patient information into a logical format to reduce the omission of important information and to facilitate consistency in the process. Aim to assess nurses' knowledge and performance regarding shift report handover. Study design: A descriptive design was used to carry out the study. Setting: This study was conducted in the Cardiac and Digestive System Center at Sohag City which affiliated to Ministry of Health (MOH). Subject: The study included (45) staff nurses. Tools: Two tools of data collection were used namely; first tool knowledge questionnaire which divided into two parts: part 1 included questions about personal characteristics of the staff nurses, part 2 consisted of multiple choice questions; and second tool an observational checklist. Results: The main study results illustrated that majority of studied nurses had very deficient total knowledge regarding shift report handover and low of performance regarding shift report handover. Conclusion: the staff nurses in the study setting have deficient knowledge and inadequate performance of communication and shift report handover. There were significant positive correlation between knowledge score and performance score. Recommendations: Hospital decision makers should create strategies to manage the quality of shift handover. The applicability of shift report handover should be supported from the hospitals administration.