Introduction: Many healthcare employees are dissatisfied with their job and working conditions. Frequently, their job dissatisfaction involves stressful situations that result in work-related illness, long-term absenteeism and lack of performance Aim: Assess the relationship between nurses work life interference and job performance Study design: A descriptive research design was used. Setting: The study conducted in General ICU at Assuit University Hospital. Subject and Method: The study subject consisted of (44) nurse working in General ICU at Assuit University Hospital. The data collected through self administered questionnaire which includes personal characteristics data, Work Interference with Personal Life Scale, and Observational Performance Checklist tool. Results: More than half of the studied nurses reported they are all the time put personal life on hold for work, job performance level of the studied nurses was satisfactory and there was no statistical significance difference between nurses' personal data and studied variables Conclusion: There was a significant negative correlation between nurses' work life interference and job performance. Recommendations: Formulate a team-based management and training for all departments, and nurse manager should use democratic leadership style in supervision to increase nurses' commitment.